Cooling system
We have the sample cooling plate from IdealVac, which has a 140ml LN2 reservoir. To fill the reservoir, asked for a quote for https://www.linde-cryo.nl/nl/producten/cryopro/dermatologische-behandeling.html - this is no longer a viable option for the setup.
Small dewars are widely available for LN2 handling and transportation. These come in many different sizes but based on the small 140 ml LN2 reservoir, we shouldn't need anything above 1 l to store LN2 intended for cooling the plate. Transfer out of the dewar can take place with several options, such as pouring through a spout and into a funnel or scooping small amounts out at a time. While pouring is a viable option since some dewars do come with a spout in place, scooping LN2 in a pre-determined-sized cryo dipper is a more careful and predictable way. This method can also use a simple funnel to prevent over spilling, though it should be considered also in the context of evapouration of the LN2 out of the LN2 trap, which could cause bubbling and splash on the sample chamber. Covering the chamber can prevent any accidental splashing.
From the module specs, we know that the LN2 reservoir can hold 140ml at a time. To cool this to liquid nitrogen temperatures (-196 celcius) requires 1L of LN2. From that point forwards, each 140ml of LN2 will take 2 hours to evaporate out of the reservoir. This can of course be topped up at any convenient time to extend this time. With this in mind, we should look to get a dewar of 2L to prevent needing to refill it.
| Product | Price | Link |
|---|---|---|
| Dewar | €237 | https://www.fishersci.nl/shop/products/benchtop-flasks/10149270 |
| Dipper | €70 | https://princetoncryo.com/default/ln2-dipper.html |