Cooling system
We have the sample cooling plate from IdealVac, which has a 140ml LN2 reservoir. To fill the reservoir, asked for a quote for https://www.linde-cryo.nl/nl/producten/cryopro/dermatologische-behandeling.html - this is no longer a viable option for the setup.
Small dewars are widely available for LN2 handling and transportation. These come in many different sizes but based on the small 140 ml LN2 reservoir, we shouldn't need anything above 1 l to store LN2 intended for cooling the plate. Transfer out of the dewar can take place with several options, such as pouring through a spout and into a funnel or scooping small amounts out at a time. While pouring is a viable option since some dewars do come with a spout in place, scooping LN2 in a pre-determined-sized cryo dipper is a more careful and predictable way. This method can also use a simple funnel to prevent over spilling, though it should be considered also in the context of evapouration of the LN2 out of the LN2 trap, which could cause bubbling and splash on the sample chamber. Covering the chamber can prevent any accidental splashing.
With this in mind, a dewar and dipper of 1 l and 200 ml respectively are linked below (the dewar also has a spout).
| Product | Price | Link |
|---|---|---|
| Dewar | €237 | https://www.fishersci.nl/shop/products/benchtop-flasks/10149270 |
| Dipper | €70 | https://princetoncryo.com/default/ln2-dipper.html |